CALLUS INDUCTION AND PLANT REGENERATION IN LISIANTHUS (EUSTOMA GRANDIFLORIUM)

This study was conducted to evaluate the effect adding hormones on callus induction and in vitro regeneration from callus of Lisianthus (Eustoma grandiflorum) by using a completely randomized design and factorial experiment. Sterile explants were placed on basal medium B5 along with different concentration of 2, 4-D and NAA under dark and light conditions. In regard to the explants location collogenesis was happened just from leaf explants. Increasing the amount of NAA, in referring the hormonal balance, lead to more collogenesis than 2, 4-D (P < 0.05). For indirect regeneration callus was subcultured in B5 medium supplemented with different concentration of Kin or BAP which applied individually or in combination with NNA, IAA and GA3. Media contains GA3 and Kin induced maximum proliferation from callus.