Efficient synthesis of globoside and isogloboside tetrasaccharides by using (beta)(1(RIGHTARROW)3)Nacetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein

The (beta)(1(RIGHTARROW)3)N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein was constructed and used in coupled enzymatic reactions to synthesize a variety of globotetraose and isoglobotetraose derivatives from the corresponding lactoside acceptors.