Parathion effects on protein synthesis in the seminiferous tubules of mice

Agropesticides impart high risks of exposure for animals including humans. This work analysed the effect of parathion upon protein synthesis in seminiferous tubules cultured in vitro from adult male CF1 mice. They were incubated with parathion solutions (0.8, 0.4, 0.04, 0.004, 0.0004 mM) for 5 h. One hour after ending incubation tritiated leucine was added to each sample. In addition, groups treated with 0.8 mM parathion were incubated for 5 h, and thereafter transferred to a parathion-free medium; tritiated leucine incorporation was measured 1, 2, or 3 h afterwards. Results of protein synthesis denote an inhibitory dose-dependent effect of parathion. This is reversed after pesticide-free incubation. Testicular protein synthesis inhibition induced by parathion may compromise male fertility in view of the fact that, in spermatogenesis, proliferation (mitosis, meiosis) and cell differentiation occurs.