Endogenous PYY and NPY mediate tonic Y1- and Y2-mediated absorption in human and mouse colon

Objective To establish the functional significance of endogenous peptide YY (PYY) and neuropeptide Y (NPY) as mediators of Y1 and Y2 absorptive tone in colonic mucosa. Methods Functional studies utilized descending colon from adult mice (wild type [WT] and peptide nulls) and ex vivo human colonic tissue (from patients undergoing bowel resections) measuring changes in basal ion transport. Peak increases in ion transport to Y1 or Y2 antagonists (BIBO3304 300 nM; BIIE0246 1 μM) were pooled (mean ± SEM) and compared using Studentʹs unpaired t test (P ≤ 0.05); some tissues received tetrodotoxin (TTX; 100 nM). PYY-positive L-cell numbers and NPY innervation were also compared. Results Y1 and Y2 tones were present in human and WT mouse colon mucosa and only the latter was TTX sensitive. Y1 tone was unchanged in NPY−/− but was not, vert, similar90% inhibited in PYY−/− and abolished in PYYNPY−/− colon mucosa. Y2 tone was reduced not, vert, similar50% in NPY−/− and PYY−/− tissues and was absent from PYYNPY−/− colon. Residual Y2 and Y1 tones present in PYY−/− mucosa were abolished by TTX. PYY ablation had no apparent effect on NPY innervation and PYY-positive cells were observed at the same frequency in NPY−/− (56.7 ± 6.8 cells/section) and WT (55.0 ± 4.6 cells/section) colons. Double knockouts lacked PYY and NPY expression, but endocrine cells and enteric nerves were present with similar frequencies to those of WT mice. Conclusion Endogenous PYY mediates Y1 absorptive tone that is epithelial in origin, whereas Y2 tone is a combination of PYY and NPY mediation.