Effects of particulate and soluble substrates on microfauna populations and treatment efficiency in activated sludge systems

To determine the effects of particulate and soluble compounds on microfauna populations and treatment efficiency in activated sludge systems, two experimental wastewater treatment plants were set up and evaluated for a period of five months. The plants were fed with pre-flocculated domestic sewage enriched with starch or glucose as model substrates of particulate and soluble organic matter, respectively. It was observed that the starch-enriched system presented lower abundance of filamentous bacteria that turned into a better sludge sedimentation. Mean sludge volume index (SVI) values for the starch and glucose-enriched systems were 54±24 and 885±845 mL g−1, respectively. Although no differences in organic matter removal were detected between the systems, nitrification and denitrification were higher in the starch-enriched system, which is likely to have been the result of its more compact flocs. The mean ammonia-N effluent concentrations for the starch and glucose-enriched systems were 4.7±5.7 and 16.2±9.7 mg L−1, respectively, whereas the nitrate-N concentrations were 20.1±10.8 and 30.8±12.2 mg L−1, respectively. Concerning microfauna analysis, ciliated protozoa—specifically, attached ciliates—were the dominant microfauna group in both treatment systems, whereas metazoa, particularly Lecanidae rotifera, were more abundant in the starch-enriched system. Lecanidae rotifera abundances above 400 ind mL−1 reduced the mean floc area from 60 to 20 mm2 without affecting sludge settleability in the starch-enriched system. Finally, the reduction in floc area caused by metazoa feeding led to effluents of lower nitrogen quality, although no differences in sludge production were detected.