Isolation and identification of an Eikelboom type 1863 strain as Acinetobacter johnsonii

Type 1863 strain RT2 was isolated by micromanipulation techniques from an activated sludge sewage treatment plant in Rome. The isolate is a coccobacillus which grows in chains. It is a strict aerobe that is oxidase negative and catalase positive, exhibits chemoorganotrophic metabolism and grows on acetate as the sole carbon source but does not grow on carbohydrates. Strain RT2 is a member of the genus Acinetobacter by the transformation assay. The 16S rDNA sequence of RT2 was determined and used to phylogenetically place the strain in the gamma subclass of the proteobacteria as Acinetobacter johnsonii.