Comparison of additives in the removal of phenolic compounds by peroxidase-catalyzed polymerization

Several additives, polyethylene glycol, gelatin and some polyelectrolytes, were studied to compare their behaviour in the removal of phenolic compounds from aqueous solution by horseradish-peroxidase-catalyzed polymerization. The effects of additives on optimum pH range, horseradish-peroxidase saving, reaction stoichiometry and minimum additive requirements were investigated. The fate of additive after reaction was also studied. The experimental results showed that all tested additives significantly reduced the horseradish-peroxidase requirement. Comparison among them revealed that polyethylene glycol was the best choice from all perspectives. It saved more peroxidase and had no negative overdose effect shown by gelatin and polyelectrolytes. At minimum polyethylene glycol dose, there was little polyethylene glycol remaining in solution after completion of reaction. However, a considerable amount of gelatin remained in solution even at the minimum gelatin dose. Also, gelatin produced more precipitate than polyethylene glycol.