Title of article :
Heart muscle cells share common neutral glycosphingolipids with Trypanosoma cruzi
Author/Authors :
Alane Beatriz Vermelho، نويسنده , , Maria de Nazareth Leal de Meirelles، نويسنده , , Mirian Claudia Pereira، نويسنده , , Gottfried Pohlentz، نويسنده , , Eliana Barreto-Bergter، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1997
Pages :
13
From page :
131
To page :
143
Abstract :
Neutral glycosphingolipids were isolated from mouse heart muscle cells and their structures were analyzed. The molecular compositions of these glycosphingolipids were examined using column chromatography, HPTLC, GC-MS and fast atom bombardment-mass spectrometry (FAB-MS). Monohexosylceramides are a mixture of glucosyl- and galactosylceramides in a ratio of 1:1, sphingosine as the long chain base and as fatty acyl groups mainly C16, C18 saturated and C22 and C24 hydroxy fatty acids. Dihexosylceramide, identified as lactosylceramide contains C18 sphingosine and C18, C20 and C22 were the major fatty acids. No evidence for the occurrence of hydroxylated fatty acids in this glycolipid could be obtained from the GC-MS data. Our results clearly demonstrated that Trypanosoma cruzi and heart muscle cells have similar glycosphingolipid structures. In addition, heart muscle cells neutral glycosphingolipids have been shown to be immunoreactive. Antibodies reactive with each of the immunogenic glycolipids from heart cells or T. cruzi epimastigotes were present in the sera of human patients with Chagas disease as detected by ELISA. These cross-reactive antigens could be involved in the Chagasic autoimmunity.
Keywords :
ELISA , Chagasic sera , Heart muscle cells , Trypanosoma cruzi , Ceramide dihexoside , Ceramide monohexoside
Journal title :
Acta Tropica
Serial Year :
1997
Journal title :
Acta Tropica
Record number :
777247
Link To Document :
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