Title of article :
Use of DNA-based diagnostic methods for human leishmaniasis in Minas Gerais, Brazil
Author/Authors :
Antero Silva Ribeiro de Andrade، نويسنده , , Rosângela F?tima Gomes، نويسنده , , Octavio Fernandes، نويسنده , , Maria Norma de Melo، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Abstract :
DNA hybridisation was used to type 26 samples from lesions of human patients from the Rio Doce Valley (Minas Gerais, Brazil) clinically diagnosed as having cutaneous leishmaniasis, using kinetoplast DNA (kDNA) cloned mini-circle probes specific for the Leishmania mexicana and Leishmania braziliensis complexes. All samples were found to belong to the L. braziliensis complex. When biopsies were pressed directly onto touch blot membranes 38.5% of the samples were positive. The positivity and specificity obtained were both 100% when cultured blotted parasites were used. The results were confirmed by polymerase chain reaction (PCR) analysis using primers specific for the L. mexicana and L. braziliensis complexes.
Keywords :
Leishmania , diagnosis , PCR , DNA hybridisation
Journal title :
Acta Tropica
Journal title :
Acta Tropica