Title of article
Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma
Author/Authors
B. M. Kotecka، نويسنده , , K. H. Rieckmann، نويسنده , , T. M. E. Davis، نويسنده , , K. T. Batty، نويسنده , , K. F. Ilett، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2003
Pages
5
From page
371
To page
375
Abstract
The study was a comparison of bioassay and HPLC analysis of artesunate (ARTS) and dihydroartemisinin (DHA) in plasma. ARTS and DHA in plasma samples from patients treated with ARTS were quantified by HPLC and expressed as DHA. DHA-equivalents in the same plasma samples were measured using a standardised parasite culture technique. DHA concentrations estimated by both methods were highly correlated (bioassay=0.96×HPLC+11.0; r2=0.92). At high concentrations (>12 000 nmol/l) bioassay sometimes overestimated DHA. Bioassay of active drug in plasma correlates well with specific chemical analysis by HPLC. ARTS and DHA appear to account for the total antimalarial activity in plasma after ARTS administration.
Keywords
malaria , Artesunate , Dihydroartemisinin , bioassay , HPLC
Journal title
Acta Tropica
Serial Year
2003
Journal title
Acta Tropica
Record number
777968
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