Analysis of the large (L) protein gene of the procine rubulavirus LPMV: identification of possible functional domains

The complete nucleotide sequence of the porcine rubulavirus LPMV (La Piedad Michoacan virus) large (L) protein gene was determined and analysed. The L mRNA was found to span 6786 nucleotides, containing one single large open reading frame (ORF), putatively encoding a polypeptide of 2551 amino acids. By aligning the amino acid sequence of the LPMV L-protein with L-proteins of a number of viruses belonging to the order mononegavirale, a high degree of similarity between the LPMV L-protein and other rubula virus L-proteins was demonstrated, extending through almost the whole protein. Additionally we could identify several regions as being highly conserved among all studied viruses of the order mononegavirale. The significance of these regions are discussed