Recombinant fowlpox virus vaccines against Australian virulent Marekʹs disease virus: gene sequence analysis and comparison of vaccine efficacy in specific pathogen free and production chickens

We have cloned and sequenced the glycoprotein genes gB, gC and gD of the Australian virulent Marekʹs disease virus (MDV) isolate Woodlands No.1. The glycoprotein gB and gC sequences were identical to the homologs of other virulent MDV type 1 strains, and the glycoprotein gD sequence contained only one unique amino acid substitution. Recombinant fowlpox viruses (rFPVs) expressing the MDV glycoprotein genes were constructed and their efficacy as vaccines was evaluated in specific pathogen free (SPF) and production chickens. Vaccination with the FPV-gB recombinant protected SPF chickens from Marekʹs disease mortality and tumour formation following challenge with virulent MDV Woodlands No.1. The degree of protection from Marekʹs disease was dependent on the vaccine dose and route of inoculation. The rFPVs expressing gC or gD did not provide protection from Marekʹs disease. A rFPV expressing both gB and gC did not provide enhanced protection in comparison with the rFPV-gB alone. The rFPV-gB vaccine failed to protect commercial chickens from MD mortality and provided little protection from tumour formation in comparison with the commercial herpesvirus of turkey (HVT) vaccine. The failure to provide protection against MD may be related to the impact of maternally derived immunity to MDV and FPV and possibly the genotype of the chickens.