Author/Authors :
L. V. Cherenova، نويسنده , , D. Y. Logunov، نويسنده , , E. V. Shashkova، نويسنده , , M. M. Shmarov، نويسنده , , L. V. Verkhovskaya، نويسنده , , G. L. Neugodova، نويسنده , , D. B. Kazansky، نويسنده , , K. K. Doronin، نويسنده , , B. S. Naroditsky ، نويسنده ,
Abstract :
In our study, a recombinant adenovirus based on the avian adenovirus CELO genome, has been constructed that contains the human interleukin-2 gene. We have shown the production of biologically active recombinant interleukin-2 in vitro (LMH and 293 cells) and in ovo (chicken embryos) infected with recombinant virus CELO-IL2. An increase in the median survival time of C57BL/6 mice carrying B16 melanoma tumors has been demonstrated after multiple intra-tumors injections of the recombinant adenovirus CELO-IL2.
Keywords :
Avian adenovirus CELO , Human interleukin-2 (IL-2) , Secreted placental alkaline phosphatase (SEAP) , Green fluorescent protein (GFP) , B16 melanoma , Recombinant adenovirus
