Author/Authors :
Yasumaru Hatanaka، نويسنده , , Makoto Hashimoto، نويسنده , , Kazuya I. -P. Jwa Hidari، نويسنده , , Yutaka Sanai، نويسنده , , Yoshitaka Nagai، نويسنده , , Yuichi Kanaoka، نويسنده ,
Abstract :
A new biotinylated lactose derivative bearing a 14C-labeled phenyldiazirine was synthesized. A convenient approach based on avidin-biotin technology was successfully applied for GM3 synthase assay and the Km value of this biotinylated photoprobe was determined as 180 μM using rat liver Golgi as the enzyme source. Further characterization revealed that this reagent could be a useful photoprobe for GM3 synthase.
