Enzymatic α(2–3)-sialylation of non-natural disaccharides with cloned sialyl-transferase

Cloned α(2–3)sialyltransferase from rat liver is used to sialylate lactosamine derivatives. The enzyme accepts a broad range of N-acetyl substituants. The residues encompass small and bulky aliphatic replacements as well as charged and sulfonamide groups.