Enzymatic fucosylation of non-natural trisaccharides with cloned fucosyltransferase VI

Non-natural sialyl-lactosamine derivatives are subjected to enzymatic fucosylation with cloned fucosyltransferase VI. The enzyme tolerates a wide range of acceptors, spanning from small and bulky aliphatic as well as charged and sulfonamide replacements of the natural N-acetyl residue.