Co-electropolymerization of a viologen oligomer and lipoamide dehydrogenase on an electrode surface. Application to cofactor regeneration

We report here a novel enzyme electrode obtained by electrochemically copolymerization of a redox enzyme with an oligomeric orgaric redox mediator on the surface of a glassy carbon electrode. This electrode serves as an efficient catalyst for the regeneration of the cofactor in enzymatic syntheses requiring NADH. The reaction shows a relatively short induction period and the rate of product formation is higher than for similar electrodes upon which the enzyme and redox mediator are physically trapped under a Nafion membrane.