Hematologic parameters study of male and female rats administrated with different concentrations of Silver Nanoparticles

ABSTRACT: Silver nanoparticles (AgNPs) are increasingly used in various products and consequentially the potential adverse effects associated with exposure to them are of concern. This study investigated the effects of AgNPs on hematologic parameters in male and female mice. Forty mice including twenty male and twenty female, were randomly divided into eight groups: C1 (male control), C2 (female control), T1, T2, T3 (male treatment) and T4, T5and T6 (female treatment). After the acclimatization period, treatment groups were gavaged with Silver Nanoparticle solution, T1 (3mg/kg), T2 (300mg/kg), T3 (1000mg/kg) and T4 (3mg/kg), T5 (300mg/kg) and T6 (1000mg/kg) for 15 days. At the end of study period, blood samples were collected from the tail vein and, immediately mixed with heparin in plastic tube. Determinations of parameters were performed using an automated biochemical analyzer (Chemistry analyzer photometer DANA-4500).Results showed that Number of RBC in T3(male,1000 mg/kg) ,T5(female,300 mg/kg) and T6(female,1000 mg/kg) decreased considerably compared to their own control (P < 0.05) .Also we observed a significantly falling in number of WBC in T2(male, 300 mg/kg),T3(male,1000 mg/kg) and likewise in all female treatment groups(T4,T5 and T6) in comparison to their respective control groups (P < 0.05). Level of Hemoglobin in all male and female groups dropped remarkably in contrast with controls (P < 0.05). Hematocrite only in T3 (male, 1000 mg/kg) and T5 (female, 300 mg/kg) exhibits significant decreasing compared to their control groups (P < 0.05). We concluded that nanosilver is able to induce vast changes in hematologic parameters in male and female mice.