Title of article
Altering the specificity of subtilisin B. Lentus by combining site-directed mutagenesis and chemical modification
Author/Authors
Per Berglund، نويسنده , , Michele R. Stabile، نويسنده , , Marvin Gold، نويسنده , , J. Bryan Jones، نويسنده , , Colin Mitchinson، نويسنده , , Richard R. Bott، نويسنده , , Thomas P. Graycar، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 1996
Pages
6
From page
2507
To page
2512
Abstract
The thiol side chain of the M222C mutant of the subtilisin from Bacillus lentus (SBL) has been chemically modified by methyl-, aminoethyl-, and sulfonatoethylthiosulfonate reagents. Introduction of charged residues into the active site of the enzyme reduced the catalytic efficiency with Suc-AAPF-pNA as the substrate, but resulted in better binding of sterically demanding boronic acid inhibitors.
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
1996
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
788386
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