Author/Authors :
Per Berglund، نويسنده , , Michele R. Stabile، نويسنده , , Marvin Gold، نويسنده , , J. Bryan Jones، نويسنده , , Colin Mitchinson، نويسنده , , Richard R. Bott، نويسنده , , Thomas P. Graycar، نويسنده ,
Abstract :
The thiol side chain of the M222C mutant of the subtilisin from Bacillus lentus (SBL) has been chemically modified by methyl-, aminoethyl-, and sulfonatoethylthiosulfonate reagents. Introduction of charged residues into the active site of the enzyme reduced the catalytic efficiency with Suc-AAPF-pNA as the substrate, but resulted in better binding of sterically demanding boronic acid inhibitors.
