Properties of fluorophores on solid phase resins; implications for screening, encoding and reaction monitoring

Fluorescent molecules covalently attached to resin beads are analysed to explore the application of on-bead fluorescence in combinatorial chemistry, and in particular: screening, encoding and reaction monitoring. The range of linearity of on-bead fluorescence is shown to be dependent on both the extent of loading and the Stokes shift of the fluorophore. Spectral deconvolution of multiple fluorophores on-bead is demonstrated.