Formation of guanosine adducts from -ascorbic acid under oxidative conditions

Ascorbic acid (AA) was incubated with guanosine under physiological conditions and the reaction was monitored by HPLC-DAD. Four reaction products were isolated and identified as two pairs of diastereomers of N2-(1-Carboxyethyl)-guanosine and N2-(1-Carboxy-3-hydroxypropyl)-guanosine, respectively. They were formed from AA in the presence of oxygen as well as from its degradation products L-dehydroascorbic acid and L-xylosone.