SDS-Subtilisin complex efficiently catalyzes synthesis of peptides in ethanol and 2-propanol

An enzymatic synthesis of tripeptide Z-Ala-Ala-Leu-pNA, tetrapeptides Z-Ala-Ala-P1-P1′-Xaa, where P1 = Leu, Trp, Met, Ala, Ile, Phe; P1′ =Phe, Ala, Leu; Xaa = pNA, NH2, pentapeptides Z-Ala-Ala-Leu-Ala-Ala-pNA and Z-Ala-Ala-Leu-Ala-Phe-pNA is described. The reactions were performed in organic solvents using SDS-subtilisin complex as a catalyst of the peptide bond synthesis.