On the preparative use of recombinant pig α(1–3)galactosyl-transferase

A series of non-natural N-acyl derivatives of lactosamine is incubated with recombinant α(1–3)galactosyl-transferase and UDP-galactose. The enzyme shows a high promiscuity towards the non-natural acceptors. It selectively transfers a galactose unit onto the 3-OH group of the terminal β-linked galactose in an α-mode to give an array of linear-B trisaccharides.