Author/Authors :
Gregor Dudziak، نويسنده , , Steffen Zeng، نويسنده , , Eric G. Berger، نويسنده , , Ricardo Gutiérrez Gallego، نويسنده , , Johannis P. Kamerling، نويسنده , , Udo Kragl، نويسنده , , Christian Wandrey، نويسنده ,
Abstract :
β-Galactosidase from bovine testes was used in a one pot reaction together with a recombinant β-1,6-GlcNAc transferase for the synthesis of GlcNAc(β1–6)GalNAc(α1-OBn) (core 6-Bn). The galactosidase, which reversibly links galactose via a (β1–3) linkage to N-acetylgalactosamine, provides the substrate for the GlcNAc transferase in situ. The synthesis was carried out with a yield >90%.
