Author/Authors :
Koji Ichinose، نويسنده , , Chandres Surti، نويسنده , , Takaaki Taguchi، نويسنده , , Francisco Malpartida، نويسنده , , Kevin I. Booker-Milburm، نويسنده , , G. Richard Stephenson، نويسنده , , Yutaka Ebizuka، نويسنده , , David A. Hopwood، نويسنده ,
Abstract :
Pyran ring formation in the biosynthesis of actinorhodin in Streptomyces coelicolor A3(2) was studied using the act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the actVI genes. The strain, CH999/pIJ5660 (pRM5 + actVI-ORF1), produced a chiral intermediate, (S)-DNPA, suggesting that the actVI-ORF1 product is a reductase determining the C-3 stereochemical centre.
