Synthesis and fluorescence properties of oregon green 514 labeled peptides

Oregon Green 514, with high photostability and a pKa of 4.8, is suitable for imaging applications. Labeling of peptide-bound resin with the car☐ylic acid form was optimized. Peptide conjugation resulted in altered fluorescence properties of the dye including a quenching of the intensity compared with that in aqueous buffers with a slight red-shift of the emission maximum.