Author/Authors :
Vladimir N. Shibaev، نويسنده , , Vladimir V. Veselovsky، نويسنده , , Antonina V. Lozanova، نويسنده , , Sergei D. Maltsev، نويسنده , , Leonid L. Danilov، نويسنده , , W. Thomas Forsee، نويسنده , , Jun-Xing Zhu، نويسنده , , Herbert C. Cheung، نويسنده , , Mark J. Jedrzejas، نويسنده ,
Abstract :
Derivatives of dolichyl phosphate (Dol-P) with 2-aminopyridine or 1-aminonaphtalene fluorophore groups at the ω-end of the chain were synthesized. These products serve as substrates for recombinant yeast Dol-P-mannose synthase. Fluorescence resonance energy transfer between a Trp residue of the enzyme and the 1-aminonaphtalene group of the Dol-P analogue was demonstrated.
