Author/Authors :
Min Wu، نويسنده , , Hong-sik Moon، نويسنده , , Asta Pirskanen and، نويسنده , , Johanna Myllyharju، نويسنده , , Kari I. Kivirikko، نويسنده , , Tadhg P. Begley and Steven E. Ealick، نويسنده ,
Abstract :
A deuteriated substrate for the human type I prolyl-4-hydroxylase was synthesized and its V/K deuterium isotope effect was determined to be 3.4 ± 0.2. This isotope effect was attributed to the uncoupled oxidation. A dehydroproline containing tetrapeptide was also found to stimulate the uncoupled oxidation.
