Author/Authors :
Atsushi Okazawa، نويسنده , , Hiroshi Maeda، نويسنده , , Eiichiro Fukusaki، نويسنده , , Yoshio Katakura، نويسنده , , Akio Kobayashi، نويسنده ,
Abstract :
DNA aptamers that bind to hematoporphyrin IX (HPIX) were isolated using an in vitro selection technique. Most aptamers obtained after the 7th and 10th rounds contained guanine-rich sequences. Binding assay using fluorescence polarization technique and structural analysis by CD spectra revealed that the parallel guanine-quartet structure of the aptamer participates in the recognition of HPIX.
