The adenine derivative of α- -LNA (α- -ribo configured locked nucleic acid): synthesis and high-affinity hybridization towards DNA, RNA, LNA and α- -LNA complementary sequences

Synthesis of a 9-mer α- -LNA (α- -ribo configured locked nucleic acid) containing three 9-(2-O,4-C-methylene-α- -ribofuranosyl)adenine nucleotide monomer(s) has been accomplished. The work involved synthesis of the bicyclic adenine nucleoside via a condensation reaction between -threo-pentofuranose derivative 1 and 6-N-benzoyladenine followed by C2′-epimerization. Hybridization studies demonstrated very strong duplex formation with 9-mer complementary DNA, RNA, LNA and α- -LNA target sequences.