Author/Authors :
Nils Brinkmann، نويسنده , , Martine Malissard، نويسنده , , Maud Ramuz، نويسنده , , Ulrike R?mer، نويسنده , , Thomas Schumacher، نويسنده , , Eric G. Berger، نويسنده , , Lothar Elling، نويسنده , , Christian Wandrey، نويسنده , , Andreas Liese، نويسنده ,
Abstract :
The α-Gal trisaccharide Galα(1→3)Galβ(1→4)GlcNAc 11 was synthesized on a homogeneously soluble polymeric support (polyethylene glycol, PEG) by use of a multi-enzyme system consisting of β-1,4-galactosyltransferase (EC 2.4.1.38), α-1,3-galactosyltransferase (EC 2.4.1.151), sucrose synthase (EC 2.4.1.13) and UDP-glucose-4-epimerase (EC 5.1.3.2). In addition workup was simplified by use of dia-ultrafiltration. Thus the advantages of classic chemistry/enzymology and solid-phase synthesis could be united in one. Subsequent hydrogenolytic cleavage afforded the free α-Gal trisaccharide.
