Author/Authors :
Ei-ichiro Fukusaki، نويسنده , , Tomohisa Hasunuma، نويسنده , , Shin-ichiro Kajiyama، نويسنده , , Atsushi Okazawa، نويسنده , , Tomohiko J. Itoh، نويسنده , , Akio Kobayashi، نويسنده ,
Abstract :
We succeeded in acquiring two DNA aptamers that selectively recognize tubulin by the SELEX method. A pool of single-stranded oligo-DNAs including a random region of 59 nucleotides was screened by SELEX for tubulin purified from calf-brain as a target. After 20 repetitions of selection round, the library converged on specific T-rich sequences. The binding activity of T-rich clones was analyzed by the SPR sensor to determine their dissociation constants to be in the order of 10 μM. ©2000 Elsevier Science Ltd. All rights reserved.
