Author/Authors :
Karen Stolze، نويسنده , , Stephen C. Holmes، نويسنده , , David J. Earnshaw، نويسنده , , Mohinder Singh، نويسنده , , Dmitry Stetsenko، نويسنده , , Donna Williams، نويسنده , , Michael J. Gait، نويسنده ,
Abstract :
Combinations of the polyamine spermine and magnesium ions synergize to dramatically enhance cleavage of the hairpin ribozyme. Certain synthetic basic tripeptides stimulate hairpin cleavage significantly at limiting magnesium ion concentration, notably the tripeptide of -diaminobutyric acid (Dab). Of a range of novel synthetic spermine–amino acid conjugates, -Dab-spermine (but not -Dab nor other amino acid conjugates) was more effective than spermine itself.
