Author/Authors :
Marco Macchia، نويسنده , , Francesca Salvetti، نويسنده , , Simone Bertini، نويسنده , , Valeria Di Bussolo، نويسنده , , Lisa Gattuso، نويسنده , , Marco Gesi، نويسنده , , Mahmoud Hamdan، نويسنده , , Karl-Norbert Klotz، نويسنده , , Teresina Laragione، نويسنده , , Antonio Lucacchini، نويسنده , , Filippo Minutolo، نويسنده , , Susanna Nencetti، نويسنده , , Chiara Papi، نويسنده , , Daniela Tuscano، نويسنده , , Claudia Martini، نويسنده ,
Abstract :
New fluorescent ligands for adenosine receptors (ARs), obtained by the insertion, in the N6 position of NECA, of NBD-moieties with linear alkyl spacers of increasing length, proved to possess a high affinity and selectivity for the A3subtype expressed in CHO cells. In fluorescence microscopy assays, compound 2d, the most active and selective for human A3-AR, permitted visualization and localization of this human receptor subtype, showing its potential suitability for internalization and trafficking studies in living cells.
