Author/Authors :
Marashi، Seyed Mahmoud Amin Seyed Mahmoud Amin نويسنده Cellular and Molecular Biology Research Center, (CMBRC), Babol University of Medical Sciences, Babol, Iran Marashi, Seyed Mahmoud Amin Seyed Mahmoud Amin , RAJABNIA، Ramazan نويسنده Babul University of Medical Sciences,Department of microbiology , , Imani Fooladi، Abbas Ali نويسنده , , Hojati، Zohreh نويسنده , , MOGHIM، SHARAREH نويسنده , , Esfahani، Bahram Nasr Bahram Nasr نويسنده Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Esfahani, Bahram Nasr Bahram Nasr
Abstract :
Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain
