Author/Authors :
Taghizadeh، Seyyed Hossein Seyyed Hossein نويسنده Department of Genetic, Faculty of Science, ShahidChamran University of Ahwaz, Ahwaz, Iran Taghizadeh, Seyyed Hossein Seyyed Hossein , Kazeminezhad، Seyyed Reza Seyyed Reza نويسنده Department of Genetic, Faculty of Science, ShahidChamran University of Ahwaz, Ahwaz, Iran Kazeminezhad, Seyyed Reza Seyyed Reza , Sefidgar، Seyyed Ali Asghar Seyyed Ali Asghar نويسنده Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran Sefidgar, Seyyed Ali Asghar Seyyed Ali Asghar , YAZDANPANAHI، NASRIN نويسنده , , Tabatabaeifar، Mohammad Amin Mohammad Amin نويسنده Department of Genetics, Faculty of Medicine, JundiShapur University of Medical Sciences, Ahwaz, Iran Tabatabaeifar, Mohammad Amin Mohammad Amin , Yousefi، Ahmad Ahmad نويسنده Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran Yousefi, Ahmad Ahmad , Lesani، Seyyed Mohammad Seyyed Mohammad نويسنده Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran Lesani, Seyyed Mohammad Seyyed Mohammad , Abolhasani، Marziyeh Marziyeh نويسنده Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran Abolhasani, Marziyeh Marziyeh , Hashemzadeh Chaleshtori، Morteza نويسنده ,
Abstract :
Hearing loss (HL) is the most frequent sensory defect affecting 1 in 1000 neonates. This can occur due to genetic or environmental causes or both. The genetic causes are very heterogenous and over 100 loci have been identified to cause autosomal recessive non - syndromic hearing loss (ARNSHL). The aim of this study was to determine the contribution of the LRTOMT gene mutations in causing ARNSHL. One hundred fifty seven pupils affected with ARNSHL from Azarbaijan Sharghi, Kordestan, Gilan and Golestan provinces, north and west of Iran, were ascertained. In this descriptive - laboratory study, the presence of LRTOMT mutations were initially checked using PCR – Single - strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) strategy. Samples with shifted bands on the gel were confirmed by DNA sequencing method. The PCR-SSCP/HA and the subsequent direct DNA sequencing showed no mutation in the population studied. We conclude that LRTOMT mutations have no role in causing sporadic deafness in the studied population. Further studies on other populations and samples could clarify the exact role of LRTOMT mutations
