Author/Authors :
Jeff Gildersleeve، نويسنده , , Jeff Janes، نويسنده , , Helle Ulrich، نويسنده , , Priscilla Yang، نويسنده , , Carlos Barbas، نويسنده , , Peter G. Schultz، نويسنده ,
Abstract :
The design and evaluation of a new genetic selection system for evolving catalytic antibodies with aldolase activity are described. Through a series of model selections, we have identified selection conditions where expression of a catalytically active antibody confers a growth advantage to Escherichia coli. In addition, we provide evidence that the growth advantage is a direct result of catalytic activity.
