Inhibition of inosine monophosphate dehydrogenase (IMPDH) by 2-[2-(Z)-Fluorovinyl]inosine 5′-Monophosphate

Inosine 5′-monophosphate dehydrogenase (IMPDH; EC 1.1.1.205) isolated from Escherichia coli B3 cells was strongly inhibited by 2-[2-(Z)-fluorovinyl]inosine 5′-monophosphate (2-FVIMP). Inhibition of IMPDH appears to be irreversible with kinact and Ki values of 0.0269 s−1 and 1.11 μM, respectively.