Author/Authors :
Xingliang Lu، نويسنده , , Dejan Nikolic، نويسنده , , Deanna J. Mitchell، نويسنده , , Richard B. Van Breemen، نويسنده , , John A. Mersfelder، نويسنده , , Russ Hille and Peter Kuhn، نويسنده , , Richard B. Silverman، نويسنده ,
Abstract :
Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H218O and 18O2, and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.
