Author/Authors :
Amarnath Natarajan، نويسنده , , Nathan Moerke، نويسنده , , Yun-Hua Fan، نويسنده , , Han Chen، نويسنده , , William J. Christ، نويسنده , , Gerhard Wagner، نويسنده , , Jose A. Halperin، نويسنده ,
Abstract :
Binding of eIF4E to the cap structure (m7GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence-polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay.
