Title of article :
Aptamer selection for the inhibition of cell adhesion with fibronectin as target
Author/Authors :
Atsushi Ogawa، نويسنده , , Naotoshi Tomita، نويسنده , , Naoko Kikuchi، نويسنده , , Shinsuke Sando، نويسنده , , Yasuhiro Aoyama، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Abstract :
An affinity column immobilizing a decapeptide H2N-RGDSPASSKP-CO2H was used to select RGD-binding aptamers from a pool of 86-mer single-strand oligodeoxynucleotides (ODNs) containing a random 40-mer sequence. The enriched library thus obtained was further selected against adsorbed fibronectin and individual aptamers were monocloned in E. coli and sequenced to give a couple of highly homologous ODNs, which indeed inhibited fibronectin-integrin mediated cell adhesion.
Keywords :
SELEX , Aptamer , cell adhesion , fibronectin , fax: +81-75-383-2767 , e-mail addresses: ssando@sbchem.kyoto-u.ac.jp , RGD.* Corresponding authors. Tel.: +81-75-383-2766 , aoyamay@sbchem.kyoto-u.ac.jp
Journal title :
Bioorganic & Medicinal Chemistry Letters
Journal title :
Bioorganic & Medicinal Chemistry Letters
