A rate determining step change in the pre-steady state of acetylcholinesterase inhibitions by 1,n-alkane-di-N-butylcarbamates

Alkane-1-N-butylcarbamate-n-ols (1–7) and 1,n-alkane-di-N-butylcarbamates (8–14) are potent pseudo-substrate inhibitors of acetylcholinesterase. For inhibitors 1–7, the pre-steady state −log Ks values and steady state −log Ki, values are linearly correlated with the tether length (N). However, for inhibitors 8–14, correlation of the −log Ks or −log Ki values against N deviates from linearity. A discontinuity of the −log Ks versus N plot, concave downwards, is indicative of a rate determining step change in the pre-steady state of acetylcholinesterase inhibitions by inhibitors 8–14.