Author/Authors :
Zhen-Dan Shi، نويسنده , , Rajeshri G. Karki، نويسنده , , Shinya Oishi، نويسنده , , Karen M. Worthy، نويسنده , , Lakshman K. Bindu، نويسنده , , Pathirage G. Dharmawardana، نويسنده , , Marc C. Nicklaus، نويسنده , , Donald P. Bottaro، نويسنده , , Robert J. Fisher، نويسنده , , Terrence R. Burke Jr.، نويسنده ,
Abstract :
Fluorescence labeling has become a general technique for studying the intracellular accumulation and localization of exogenously administered materials. Reported herein is a low nanomolar affinity Grb2 SH2 domain-binding antagonist that utilizes the environmentally-sensitive nitrobenzoxadiazole (NBD) fluorophore as a naphthyl replacement. This novel agent should serve as a useful tool to visualize the actions of this class of Grb2 SH2 domain-binding antagonists in whole cell systems.
