Author/Authors :
Kazunori Yamamoto، نويسنده , , Hitoshi Yamaoka and Naoki Watanabe، نويسنده , , Hiroko Matsuda، نويسنده , , Keiichiro Oohara، نويسنده , , Tomoyuki Araya، نويسنده , , Masaru Hashimoto، نويسنده , , Kazuo Miyairi، نويسنده , , Isao Okazaki، نويسنده , , Minoru Saito، نويسنده , , Tetsuya Shimizu، نويسنده , , Hiroaki Kato، نويسنده , , Toshikatsu Okuno، نويسنده ,
Abstract :
A sulfur-substituted analogue of trigalacturonic acid (3) was synthesized. The synthesis features the application of 3-cyano-3-(tert-butyldimethylsilyl)oxypropylthioether (CSP) as a novel protective group for thiols. This analogue was designed with the expectation that it would be a stable analogous substrate for endo-polygalacturonase isolated from Stereum purpureum based on computer modeling experiments. Surface plasmon resonance experiments revealed that 3 forms a stable complex with the target enzyme.
