Title of article
Macrolactonization to 10-deoxymethynolide catalyzed by the recombinant thioesterase of the picromycin/methymycin polyketide synthase
Author/Authors
Weiguo He، نويسنده , , Jiaquan Wu، نويسنده , , Chaitan Khosla and Robert M. Stroud، نويسنده , , David E. Cane، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2006
Pages
4
From page
391
To page
394
Abstract
The recombinant thioesterase (TE) domain of the picromycin/methymycin synthase (PICS) catalyzes the macrolactonization of 3, the N-acetylcysteamine thioester of seco-10-deoxymethynolide to generate 10-deoxymethynolide (1) with high efficiency. By contrast, 4, the 7-dihydro derivative of seco-thioester 3, undergoes exclusive hydrolysis by PICS TE to seco-acid 5. The recombinant TE domain of 6-deoxyerythronolide B synthase (DEBS TE) shows the same reaction specificity as PICS TE, but with significantly lower activity.
Keywords
biosynthesis , synthase , antibiotics , Thioesterase , macrolactonization , polyketides
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2006
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
796382
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