A PNA–DNA hybridization chip approach for the detection of β-secretase activity

Developed was the addressable chip technology based on the PNA–DNA complementary hybridization equipped with short seven-mer PNA-encoded peptides that can be a versatile scaffold to monitor on-chip immunoassays. We also developed and validated a methodology to perform β-secretase enzyme assay with a highly sensitive fashion, resulting that a peptide substrate tethering dual fluorescent probes allowed us to detect β-secretase activity 10 times more sensitively than assays in solution.