Title of article
Genetic introduction of a diketone-containing amino acid into proteins
Author/Authors
Huaqiang Zeng، نويسنده , , Jianming Xie، نويسنده , , Peter G. Schultz، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2006
Pages
4
From page
5356
To page
5359
Abstract
An orthogonal tRNA/aminoacyl-tRNA synthetase pair was evolved that makes possible the site-specific incorporation of an unnatural amino acid bearing a β-diketone side chain into proteins in Escherichia coli with high translational efficiency and fidelity. Proteins containing this unnatural amino acid can be efficiently and selectively modified with hydroxylamine derivatives of fluorophores and other biophysical probes.
Keywords
Bioorganic chemistry , In vivo selection , Diketone-containing amino acid , aminoacyl-tRNA synthetase , protein modification
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2006
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
797386
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