Title of article :
Degradation of DNA by bisulfite treatment
Author/Authors :
Kazuo Tanaka، نويسنده , , Akimitsu Okamoto، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Abstract :
A significant level of target degradation was caused by bisulfite treatment for methylcytosine-selective hydrolysis. The depyrimidination proceeded via addition of bisulfite to pyrimidines in DNA. The quantification with real-time PCR after conventional bisulfite treatment showed a large decrease in the amount of full-length DNA.
Keywords :
DNA , Cytosine , Methylcytosine , Bisulfite , Epigenotyping , Hydrolysis
Journal title :
Bioorganic & Medicinal Chemistry Letters
Journal title :
Bioorganic & Medicinal Chemistry Letters
