Author/Authors :
Shubhasish Mukherjee، نويسنده , , Carolyn A. Robinson، نويسنده , , Andrew G. Howe، نويسنده , , Tali Mazor، نويسنده , , Peter A. Wood، نويسنده , , Sameer Urgaonkar، نويسنده , , Alan M. Hebert، نويسنده , , Debabrata RayChaudhuri، نويسنده , , Jared T. Shaw، نويسنده ,
Abstract :
A new small molecule inhibitor of bacterial cell division has been discovered using a high-throughput screen in Escherichia coli. Although the lead screening hit (534F6) exhibited modest inhibition of the GTPase activity of FtsZ (20 ± 5% at 100 μM of compound), a primary target for bacterial cell division inhibitors, several analogs caused potent bacterial growth inhibition with negligible antagonism of FtsZ GTPase activity. A library of analogs has been prepared and several alkyne-tagged photoaffinity probes have been synthesized for use in experiments to elucidate the primary target of this compound.
