Author/Authors :
Birte Vester، نويسنده , , Anne Marie Boel، نويسنده , , Sune Lobedanz، نويسنده , , B. Ravindra Babu، نويسنده , , Michael Raunkj?r، نويسنده , , Dorthe Lindegaard، نويسنده , , Raunak، نويسنده , , Patrick J. Hrdlicka، نويسنده , , Torben H?jland، نويسنده , , Pawan K. Sharma، نويسنده , , Surender Kumar Pal، نويسنده , , Poul Nielsen، نويسنده , , Jesper Wengel، نويسنده ,
Abstract :
Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3–ON12 and ON15–ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.
Keywords :
Modified oligonucleotide , RNase H cleavage , antisense oligonucleotide , Antisense technology , Modified nucleotide
